INSERTIONAL SEQUENCE PRIMERS FOR BORDETELLA-PERTUSSIS DIAGNOSTIC POLYMERASE CHAIN-REACTION DIFFERENTIATE STRAINS OF PSEUDOMONAS-CEPACIA

被引：6

作者：

CIMOLAI, N

TROMBLEY, C

机构：

[1] UNIV BRITISH COLUMBIA, DEPT PATHOL, VANCOUVER, BC, CANADA

[2] UNIV BRITISH COLUMBIA, MED LAB, VANCOUVER, BC, CANADA

来源：

JOURNAL OF INFECTIOUS DISEASES | 1995年 / 172卷 / 01期

关键词：

D O I：

10.1093/infdis/172.1.293

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Insertion sequence primers originally intended to amplify singular specific product for the rapid diagnosis of Bordetella pertussis respiratory infection were used to differentiate strains fo Pseudomonas (Burkholderia) cepacia. A modified sample preparation of proteinase K treatment and boiling ws used in lieu of DNA extraction. The method was simple, rapid and reproducible. This scheme identified 10 variations among 35 strains. Repeat strains from patients with cystic fibrosis and epidemiologically linked strains from an infection associated with a jet gun injection device were homologous in each setting.

引用

页码：293 / 295

页数：3

共 11 条

[1] ARBITRARILY PRIMED POLYMERASE CHAIN-REACTION AS A RAPID METHOD TO DIFFERENTIATE CROSSED FROM INDEPENDENT PSEUDOMONAS-CEPACIA INFECTIONS IN CYSTIC-FIBROSIS PATIENTS [J].