FLUORESCENCE DECAY TIME CHARACTERISTICS OF COMPLEX BETWEEN ETHIDIUM BROMIDE AND NUCLEIC ACIDS

被引：56

作者：

BURNS, VWF

机构：

[1] Department of Physiological Sciences, University of California, Davis

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 133卷 / 02期

关键词：

D O I：

10.1016/0003-9861(69)90471-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

When ethidium bromide is complexed to DNA or RNA, ultraviolet light absorbed by the nucleic acid can migrate to ethidium, which fluoresces. The time intervening between absorption and emission has been measured. The times increase with the nucleotide-to-ethidium ratio and reach maxima of 23 nsec for DNA and 19.5 nsec for RNA. Polarization measurements indicate that energy is transferred to rigidly bound ethidium only. © 1969.

引用

页码：420 / &

共 8 条

[1] FLUORESCENCE DECAY TIMES - PROTEINS COENZYMES AND OTHER COMPOUNDS IN WATER [J].