REACTION OF CATECHOLAMINES WITH HYDROXYLAMINE AND ITS APPLICATION TO ASSAY OF CATECHOL O-METHYL TRANSFERASE

被引：8

作者：

ABDELLATIF, AA

机构：

[1] Department of Biochemistry, Medical College of Georgia, Augusta

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 29卷 / 03期

关键词：

D O I：

10.1016/0003-2697(69)90331-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Serotonin, norepinephrine, and epinephrine react rapidly with hydroxylamine in acidic or bisic medium and form characteristic colored complexes upon addition of the ferric chloride reagent. These complexes were found to be stable with time, they absorb visible light maximally in the range λ 520 to 540 mμ, where little or no absorption is shown with ferric chloride, and the optical density of their color is a function of the neurohormone concentration. A simple and sensitive assay is described for the enzyme catechol O-methyl transferase, based on disappearance of the colored complex upon methylation of the reactive hydroxyl group of the catecholamine. © 1969.

引用

页码：468 / +

页数：1

共 9 条

[1] A RAPID AND SENSITIVE PROCEDURE FOR COMBINED ASSAY OF NORADRENALINE DOPAMINE AND SEROTONIN IN A SINGLE BRAIN SAMPLE [J].