DISTINCT SINGLE-COPY SEQUENCE SETS IN SEA-URCHIN NUCLEAR RNAS

被引：13

作者：

ERNST, SG ^{[1
]}

BRITTEN, RJ ^{[1
]}

DAVIDSON, EH ^{[1
]}

机构：

[1] CALTECH,CORONA DEL MAR,CA 92625

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 05期

关键词：

D O I：

10.1073/pnas.76.5.2209

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The purpose of this study was to determine whether nuclear RNAs (nRNA) of sea urchin embryos and adult tissues contain identical or partially distinct sets of single-copy sequence transcripts. A DNA tracer was prepared consisting mainly of sequences absent from gastrula nRNA; 3.6% of this tracer reacted with adult intestine nRNA but not with gastrula nRNA. The existence of a differentially transcribed DNA fraction was verified by its partial purification and rehybridization to intestine and gastrula nRNAs. About one-third of the genomic single-copy sequence is represented in both nRNAs, or about 2 x 10(8) nucleotides. The differentially transcribed portion of the single-copy genome identified in this work includes about 3.5 x 10(7) nucleotides.

引用

页码：2209 / 2212

页数：4

共 23 条

[1] ANALYSIS OF COMPLEXITY AND DIVERSITY OF MESSENGER-RNA FROM CHICKEN LIVER AND OVIDUCT [J].