A NOVEL AND SENSITIVE ASSAY FOR PHOSPHOLIPASE-D IN INTACT-CELLS

被引：49

作者：

RANDALL, RW

BONSER, RW

THOMPSON, NT

GARLAND, LG

机构：

[1] Department of Biochemical Sciences, The Wellcome Research Laboratories, Beckenham

来源：

FEBS LETTERS | 1990年 / 264卷 / 01期

关键词：

Neutrophil; Opsonised-zymosan; Phospholipase D; Platelet; Transphosphatidylation;

D O I：

10.1016/0014-5793(90)80772-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel and sensitive assay for phospholipase D (PLD) that measures the incorporation of high specific activity [3H]butan-1-ol into [3H]phosphatidylbutanol has been developed. The assay has been used to measure PLD activation in human neutrophils and platelets. Both the chemotactic peptide fMet-Leu-Phe and opsonised-zymosan stimulated PLD in the human neutrophil. In the platelet, PLD was stimulated by thrombin and collagen but responses were small and only occurred at high agonist concentrations. This assay has a number of advantages over existing techniques and should be valuable for investigating PLD activation in a variety of isolated cells and possibly intact tissues. © 1990.

引用

页码：87 / 90

页数：4

共 26 条

[1]

BARTLETT GR, 1959, J BIOL CHEM, V234, P466

[2]

BECKER EL, 1979, AM J PATHOL, V95, P81

[3] PHOSPHOLIPASE D ACTIVATION BY THE MITOGENS PLATELET-DERIVED GROWTH-FACTOR AND 12-O-TETRADECANOYLPHORBOL 13-ACETATE IN NIH-3T3 CELLS [J].