ATP CITRATE LYASE FROM GERMINATING CASTOR BEAN ENDOSPERM - LOCALIZATION AND SOME PROPERTIES

被引：36

作者：

FRITSCH, H ^{[1
]}

BEEVERS, H ^{[1
]}

机构：

[1] UNIV CALIF SANTA CRUZ, THIMANN LABS, SANTA CRUZ, CA 95064 USA

来源：

PLANT PHYSIOLOGY | 1979年 / 63卷 / 04期

关键词：

D O I：

10.1104/pp.63.4.687

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

ATP citrate lyase (EC 4.1.3.8) was found in crude extracts from endosperm tissue of germinating castor bean. It shows its maximum activity in 4-5 day old seedlings. A strict requirement for coenzyme A and ATP was demonstrated. The pH optimum for the reaction is around 7.5. The unstable enzyme can be stabilized by freezing and addition of citrate and glycerol. (-)-Hydroxycitrate is a potent inhibitor. The MW is about 400,000. The ATP citrate lyase is localized in the plastids, where it may play a role in providing acetyl coenzyme A for lipid biosynthesis.

引用

页码：687 / 691

页数：5

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