ACETYLCHOLINE INDUCES VOLTAGE-INDEPENDENT INCREASE OF CYTOSOLIC CALCIUM IN MOUSE MYOTUBES

被引:35
作者
GIOVANELLI, A
GRASSI, F
MATTEI, E
MILEO, AM
EUSEBI, F
机构
[1] IST REGINA ELENA,CTR RIC SPERIMENTALE,BIOFIS LAB,VIA MESSI DORO 156,I-00158 ROME,ITALY
[2] UNIV LAQUILA,DIPARTIMENTO MED SPERIMENTALE,I-67100 LAQUILA,ITALY
[3] CNR,IST TECNOL BIOMED,I-00161 ROME,ITALY
关键词
ACETYLCHOLINE RECEPTORS; CA2+ TRANSIENTS; INOSITOL 1,4,5-TRIPHOSPHATE;
D O I
10.1073/pnas.88.22.10069
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Electrophysiological, biochemical, and Ca2+ imaging studies of cultured mouse myotubes were used to investigate whether the neurotransmitter acetylcholine causes an increase in intracellular Ca2+ concentration ([Ca2+]i) through activation of a second messenger system. Bath applications of acetylcholine to myotubes (i) elicited a significant membrane current even in a Na+-free Ca2+ medium, when the current was carried mainly by calcium ions; (ii) caused a rapid and transient cytosolic accumulation of inositol 1,4,5-trisphosphate; (iii) evoked a conspicuous alpha-bungarotoxin-sensitive long-lasting [Ca2+]i enhancement even in the presence of Cd2+; and (iv) transiently increased [Ca2+]i when cells were equilibrated in a Ca2+-free atropine-containing medium. We propose that, in addition to opening ion channels, the nicotinic action of acetylcholine on the muscle cell membrane increases [Ca2+]i through activation of the inositol 1,4,5-trisphosphate second messenger system and mobilization of Ca2+ from intracellular stores.
引用
收藏
页码:10069 / 10073
页数:5
相关论文
共 27 条
  • [1] ACETYLCHOLINE STIMULATES PHOSPHATIDYLINOSITOL TURNOVER AT NICOTINIC RECEPTORS OF CULTURED MYOTUBES
    ADAMO, S
    ZANI, BM
    NERVI, C
    SENNI, MI
    MOLINARO, M
    EUSEBI, F
    [J]. FEBS LETTERS, 1985, 190 (01) : 161 - 164
  • [2] BERRIDGE MJ, 1987, ANNU REV BIOCHEM, V56, P159, DOI 10.1146/annurev.bi.56.070187.001111
  • [3] INOSITOL PHOSPHATES AND CELL SIGNALING
    BERRIDGE, MJ
    IRVINE, RF
    [J]. NATURE, 1989, 341 (6239) : 197 - 205
  • [4] BREGESTOVSKI PD, 1979, NATURE, V284, P560
  • [5] BYERLY L, 1988, CALCIUM ION CHANNEL, P3
  • [6] MASS CHANGES OF INOSITOL(1,4,5) TRISPHOSPHATE IN TRACHEALIS MUSCLE FOLLOWING AGONIST STIMULATION
    CHILVERS, ER
    CHALLISS, RAJ
    BARNES, PJ
    NAHORSKI, SR
    [J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 1989, 164 (03) : 587 - 590
  • [7] INTRACELLULAR CA-2+ ACTIVATES PHOSPHOLIPASE-C
    EBERHARD, DA
    HOLZ, RW
    [J]. TRENDS IN NEUROSCIENCES, 1988, 11 (12) : 517 - 520
  • [8] ACETYLCHOLINE MAY REGULATE ITS OWN NICOTINIC RECEPTOR-CHANNEL THROUGH THE C-KINASE SYSTEM
    EUSEBI, F
    GRASSI, F
    NERVI, C
    CAPORALE, C
    ADAMO, S
    ZANI, BM
    MOLINARO, M
    [J]. PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1987, 230 (1260): : 355 - 365
  • [9] AGENTS THAT ACTIVATE PROTEIN KINASE-C REDUCE ACETYLCHOLINE SENSITIVITY IN CULTURED MYOTUBES
    EUSEBI, F
    MOLINARO, M
    ZANI, BM
    [J]. JOURNAL OF CELL BIOLOGY, 1985, 100 (04) : 1339 - 1342
  • [10] REGULATION OF ACETYLCHOLINE-RECEPTOR DESENSITIZATION IN MOUSE MYOTUBES BY CYTOSOLIC CYCLIC-AMP
    GIOVANNELLI, A
    FARINI, D
    GAUZZI, MC
    ALEMA, S
    EUSEBI, F
    [J]. CELLULAR SIGNALLING, 1990, 2 (04) : 347 - 352