EXPRESSION IN ESCHERICHIA-COLI AND CHARACTERIZATION OF A RECOMBINANT FE-7 FERREDOXIN OF RHODOBACTER-CAPSULATUS

被引：19

作者：

JOUANNEAU, Y ^{[1
]}

DUPORT, C ^{[1
]}

MEYER, C ^{[1
]}

GAILLARD, J ^{[1
]}

机构：

[1] CEN,DRFMC,SESM,SPECTROSCOPIE COMPLEXES POLYMET & MET PROT LAB,F-38041 GRENOBLE,FRANCE

来源：

BIOCHEMICAL JOURNAL | 1992年 / 286卷

关键词：

D O I：

10.1042/bj2860269

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 7Fe ferredoxin of Rhodobacter capsulatus (FdII) could be expressed in Escherichia coli by cloning the fdxA gene coding for FdII downstream from the lac promoter. The expressed recombinant ferredoxin appeared as a brown protein which was specifically recognized in E. coli cell-free extracts by anti-FdII serum. The purified recombinant ferredoxin was indistinguishable from R. capsulatus FdII on the basis of its molecular, redox and spectroscopic properties. These results indicate that the [3Fe-4S] and [4Fe-4S] clusters were correctly inserted into the recombinant ferredoxin.

引用

页码：269 / 273

页数：5

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