CONTRACTILE PROTEIN GENE-EXPRESSION IN SERUM-FREE CULTURED ADULT-RAT CARDIAC MYOCYTES

被引：14

作者：

DUBUS, I

RAPPAPORT, L

BARRIEUX, A

LOMPRE, AM

SCHWARTZ, K

SAMUEL, JL

机构：

[1] HOP LARIBOISIERE,INSERM,U127,41 BD CHAPELLE,F-75475 PARIS 10,FRANCE

[2] ECOLE POLYTECH,INSERM,U275,F-91120 PALAISEAU,FRANCE

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1993年 / 423卷 / 5-6期

关键词：

BETA-MYOSIN HEAVY CHAIN MESSENGER RNA; ALPHA-SKELETAL ACTIN MESSENGER RNA; DOT-BLOT ANALYSIS;

D O I：

10.1007/BF00374941

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

The effects of two adhesion substrates (serum and laminin) and time in culture on the expression of genes encoding myosin heavy chain (MHC) isoforms and alpha-skeletal actin were analysed in myocytes isolated from adult rat heart and maintained in serum-free culture. Relative messenger ribonucleic acid (mRNA) abundances were quantitated by dot-blot analysis. Gene expression was not influenced by the substrate used. Time in culture induced a decrease in total mRNA abundance and an up-regulation of beta-MHC and alpha-skeletal actin genes. It is proposed that atrophy of adult myocytes is associated with a pattern of gene expression similar to the fetal program.

引用

收藏

页码：455 / 461

页数：7

相关论文

共 29 条

[1] GENETIC-ANALYSIS OF THE INTERACTION BETWEEN CARDIAC AND SKELETAL ACTIN GENE-EXPRESSION IN STRIATED-MUSCLE OF THE MOUSE [J].

ALONSO, S ;

GARNER, I ;

VANDEKERCKHOVE, J ;

BUCKINGHAM, M .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 211 (04) :727-738

[2]

Bergmeyer HU, 1974, METHOD ENZYMAT AN, P574

[3] SKELETAL ACTIN MESSENGER-RNA INCREASES IN THE HUMAN HEART DURING ONTOGENIC DEVELOPMENT AND IS THE MAJOR ISOFORM OF CONTROL AND FAILING ADULT HEARTS [J].

BOHELER, KR ;

CARRIER, L ;

DELABASTIE, D ;

ALLEN, PD ;

KOMAJDA, M ;

MERCADIER, JJ ;

SCHWARTZ, K .

JOURNAL OF CLINICAL INVESTIGATION, 1991, 88 (01) :323-330

[4] RECOGNITION OF EXTRACELLULAR-MATRIX COMPONENTS BY NEONATAL AND ADULT CARDIAC MYOCYTES [J].

BORG, TK ;

RUBIN, K ;

LUNDGREN, E ;

BORG, K ;

OBRINK, B .

DEVELOPMENTAL BIOLOGY, 1984, 104 (01) :86-96

[5] DIFFERENTIATION OF ADULT-RAT CARDIAC MYOCYTES IN CELL-CULTURE [J].

BUGAISKY, LB ;

ZAK, R .

CIRCULATION RESEARCH, 1989, 64 (03) :493-500

[6] EXPRESSION OF THE SARCOMERIC ACTIN ISOGENES IN THE RAT-HEART WITH DEVELOPMENT AND SENESCENCE [J].

CARRIER, L ;

BOHELER, KR ;

CHASSAGNE, C ;

DELABASTIE, D ;

WISNEWSKY, C ;

LAKATTA, EG ;

SCHWARTZ, K .

CIRCULATION RESEARCH, 1992, 70 (05) :999-1005

[7]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[8] SYNTHESIS OF STRESS PROTEINS IN RAT CARDIAC MYOCYTES 2-4 DAYS AFTER IMPOSITION OF HEMODYNAMIC OVERLOAD [J].

DELCAYRE, C ;

SAMUEL, JL ;

MAROTTE, F ;

BESTBELPOMME, M ;

MERCADIER, JJ ;

RAPPAPORT, L .

JOURNAL OF CLINICAL INVESTIGATION, 1988, 82 (02) :460-468

[9] BETA-ADRENERGIC AGONISTS STIMULATE THE SYNTHESIS OF NONCONTRACTILE BUT NOT CONTRACTILE PROTEINS IN CULTURED MYOCYTES ISOLATED FROM ADULT-RAT HEART [J].

DUBUS, I ;

SAMUEL, JL ;

MAROTTE, F ;

DELCAYRE, C ;

RAPPAPORT, L .

CIRCULATION RESEARCH, 1990, 66 (03) :867-874

[10] HETEROGENEOUS DISTRIBUTION OF ALPHA-MHC AND BETA-MHC MESSENGER-RNAS WITHIN ADULT-RAT CARDIAC MYOCYTE POPULATION - A QUANTITATIVE INSITU HYBRIDIZATION STUDY [J].

DUBUS, I ;

NALLET, O ;

MERCADIER, A ;

LUCAS, O ;

RAPPAPORT, L ;

SAMUEL, JL .

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1992, 24 :S11-S11