POLYMERASE CHAIN-REACTION FOR THE DIAGNOSIS OF CANDIDEMIA

被引：105

作者：

KAN, VL

机构：

[1] Infectious Diseases Section, Department of Medicine, VA Medical Center, Washington, DC

来源：

JOURNAL OF INFECTIOUS DISEASES | 1993年 / 168卷 / 03期

关键词：

D O I：

10.1093/infdis/168.3.779

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A PCR assay was developed for the diagnosis of candidemia. Primers were selected to amplify a 158-bp segment from the Candida actin gene that was detected by hybridization with a P-32-labeled oligomer probe. The lower limit of detection was DNA extracted from 10 Candida yeasts/sample, or 25 fg of purified Candida albicans DNA. This PCR was specific for medically important Candida species. Circulating Candida DNA was detected by this PCR from plasma of mice with induced candidemia and from sera in 11 (79%) of 14 patients with blood cultures positive for Candida species. This PCR may offer a sensitive method for diagnosis of candidemia.

引用

页码：779 / 783

页数：5

共 17 条

[1] BROWN WB, 1977, STATISTICS BIOMEDICA, P150
[2] BUCHMAN TG, 1990, SURGERY, V108, P338
[3] Chaconas G, 1980, Methods Enzymol, V65, P75
[4] CANDIDEMIA IN A TERTIARY CARE HOSPITAL - EPIDEMIOLOGY, RISK-FACTORS, AND PREDICTORS OF MORTALITY
FRASER, VJ
JONES, M
DUNKEL, J
STORFER, S
MEDOFF, G
DUNAGAN, WC
[J]. CLINICAL INFECTIOUS DISEASES, 1992, 15 (03) : 414 - 421
[5] SMALL SUBUNIT RIBOSOMAL-RNA OF BLASTOMYCES-DERMATITIDIS - SEQUENCE AND PHYLOGENETIC ANALYSIS
GEBER, A
HIGGINS, DE
WATERS, AP
BENNETT, JE
MCCUTCHAN, TF
[J]. JOURNAL OF GENERAL MICROBIOLOGY, 1992, 138 : 395 - 399
[6] PLOIDY AND DNA CONTENT OF CANDIDA-STELLATOIDEA CELLS
KWONCHUNG, KJ
WICKES, BL
WHELAN, WL
[J]. INFECTION AND IMMUNITY, 1987, 55 (12) : 3207 - 3208
[7] COLLABORATIVE EVALUATION OF ANTIGEN-DETECTION BY A COMMERCIAL LATEX AGGLUTINATION-TEST AND ENZYME-IMMUNOASSAY IN THE DIAGNOSIS OF INVASIVE CANDIDIASIS
LEMIEUX, C
STGERMAIN, G
VINCELETTE, J
KAUFMAN, L
DEREPENTIGNY, L
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (02) : 249 - 253
[8] SEQUENCE OF THE CANDIDA-ALBICANS GENE ENCODING ACTIN
LOSBERGER, C
ERNST, JF
[J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (22) : 9488 - 9488
[9] MOLECULAR PROBE FOR IDENTIFICATION OF MEDICALLY IMPORTANT CANDIDA SPECIES AND TORULOPSIS-GLABRATA
MASON, MM
LASKER, BA
RIGGSBY, WS
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (03) : 563 - 566
[10] DIRECT PCR FROM WHOLE-BLOOD, WITHOUT DNA EXTRACTION
MERCIER, B
GAUCHER, C
FEUGEAS, O
MAZURIER, C
[J]. NUCLEIC ACIDS RESEARCH, 1990, 18 (19) : 5908 - 5908

← 1 2 →