HUMAN-MALIGNANT ASTROCYTES EXPRESS MACROPHAGE PHENOTYPE

被引:35
作者
LEENSTRA, S
DAS, PK
TROOST, D
DEBOER, OJ
BOSCH, DA
机构
[1] UNIV AMSTERDAM,DEPT PATHOL & DERMATOL,AMSTERDAM,NETHERLANDS
[2] UNIV AMSTERDAM,ACAD MED CTR,DEPT NEUROPATHOL,1105 AZ AMSTERDAM,NETHERLANDS
关键词
BRAIN NEOPLASM; GLIOMA; ASTROCYTE; IMMUNOHISTOCHEMISTRY; CELL CULTURE; MACROPHAGE;
D O I
10.1016/0165-5728(94)00128-B
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Six well-characterized specimens of cultured astrocytoma cells were investigated with a panel of macrophage markers. Our results show that the macrophage markers OKM-1(CD11b), OKM5(CD36), EBM11(CD68), HAM56, Factor 13, alpha-1-antichymotrypsin, alpha-1-antitrypsin, ferritin and lysozyme are clearly reactive to neoplastic astrocytes whereas astrocytes in normal brain specimens are not reactive. In order to obtain further confirmation concerning the reactivity of tumor cells in vivo, we simultaneously measured by flow cytometric analysis DNA content and HAM56 immunoreactivity in a freshly obtained tumor specimen. In this experiment we found a marked reactivity of aneuploid cells to HAM56. The macrophage phenotype of malignant astrocytes may reflect a similarity in functions of these cells and tumor-associated macrophages which promote tumor growth via the production of growth factors and angiogenic factors. Furthermore, our findings implicate that demonstration of macrophages within malignant astrocytomas by using macrophage-specific antibodies must be cautiously considered.
引用
收藏
页码:17 / 25
页数:9
相关论文
共 29 条
[1]   CYTOGENETICS OF HUMAN BRAIN-TUMORS [J].
BIGNER, SH ;
MARK, J ;
BIGNER, DD .
CANCER GENETICS AND CYTOGENETICS, 1990, 47 (02) :141-154
[2]  
BUDKA H, 1985, J NEUROONCOL, V173, P172
[3]   EXPRESSION OF HLA-DR AND COMMON ACUTE LYMPHOBLASTIC-LEUKEMIA ANTIGENS ON GLIOMA-CELLS [J].
CARREL, S ;
DETRIBOLET, N ;
GROSS, N .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1982, 12 (04) :354-357
[4]  
CUSIMANO G, 1990, ACTA NEUROL SCAND, V81, P215
[5]  
FONTANA A, 1983, NATURE, V307, P273
[6]  
FRANKLIN WA, 1986, LAB INVEST, V54, P322
[7]   MACROPHAGE MARKERS IN DIAGNOSTIC NEUROPATHOLOGY [J].
HULETTE, CM ;
DOWNEY, BT ;
BURGER, PC .
AMERICAN JOURNAL OF SURGICAL PATHOLOGY, 1992, 16 (05) :493-499
[8]   A CYTOGENETIC STUDY OF 53 HUMAN GLIOMAS [J].
JENKINS, RB ;
KIMMEL, DW ;
MOERTEL, CA ;
SCHULTZ, CG ;
SCHEITHAUER, BW ;
KELLY, PJ ;
DEWALD, GW .
CANCER GENETICS AND CYTOGENETICS, 1989, 39 (02) :253-279
[9]  
JENNINGS MT, 1989, J NEUROIMMUNOL, V25, P27
[10]  
Kleihues P., 1993, HISTOLOGICAL TYPING