DNA POLYMERASE ACTIVITY IN METHOTREXATE PLUS UTIDINE TREATED TETRAHYMENA

被引：33

作者：

WESTERGA.O

PEARLMAN, RE

机构：

[1] Biological Institute, the Carlsberg Foundation, Copenhagen

来源：

EXPERIMENTAL CELL RESEARCH | 1969年 / 54卷 / 03期

关键词：

D O I：

10.1016/0014-4827(69)90207-9

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The specific activity of DNA polymerase of Tetrahymena can be increased by treatment of the cells with methotrexate plus uridine. The increase does not occur in the presence of protein synthesis inhibitors and is also affected by an inhibitor of RNA synthesis. The specific activity of DNA polymerase can also be increased by treatment of Tetrahymena with ultraviolet irradiation. It is suggested that the increase in enzymic specific activity represents de novo synthesis of DNA polymerase in response to agents which cause repairable damage to Tetrahymena DNA. © 1969.

引用

页码：309 / &

共 25 条

[1]

BOLLUM FJ, 1959, J BIOL CHEM, V234, P2733

[2] REPAIR OF DAMAGED DNA IN A EUCARYOTIC CELL - TETRAHYMENA PYRIFORMIS [J].