LARGE SCALE PRODUCTION OF TRANSFER RIBONUCLEIC ACIDS FROM E-COLI K-12 MO7

An engineering‐scale procedure for the recovery of 300–400 g batches of mixed transfer ribonucleic acids is described. Semicontinuous growth of E. coli K‐12 MO7 yielded 77 kg of harvested cells in four days. Phenol extraction and ethanol precipitation recovered a crude tRNA material that was further purified by DKAE‐cellulose chromatography in runs of 1 × 106 A260 units each on a 6 × 30 in. column using a 240 1, gradient elution. The purified tRNAs were partially concentrated and resolved into three groups. Copyright © 1969 John Wiley & Sons, Inc.