CHROMOSOMAL ABERRATION TESTS ON 29 CHEMICALS COMBINED WITH S9 MIX INVITRO

被引:157
作者
MATSUOKA, A
HAYASHI, M
ISHIDATE, M
机构
[1] Division of Mutagenesis, Biological Safety Research Center, National Institute of Hygienic Sciences, Setagaya-ku, Tokyo, 158
来源
MUTATION RESEARCH | 1979年 / 66卷 / 03期
关键词
D O I
10.1016/0165-1218(79)90089-2
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A metabolic activation system with rat-liver microsome fraction plus cofactors (S9 mix) was applied to chromosomal aberration tests in vitro for the screening of chemical mutagens or carcinogens in the environment. Dialkylnitrosamines only induced chromosomal aberrations in Chinese hamsters cells (CHL) when treated with S9 mix. The incidence of chromosomal aberrations in CHL varied with experimental conditions, e.g. incubation time, recovery time, components of S9 mix and inducers used for preparation of S9, For dimethylnitrosamine (DMN), the maximal incidence was obtained when the cells were incubated with S9 mix for 3 h and harvested 24 h after treatment. Therefore, this system (3 h incubation and 24 h recovery) was routinely applied to further screening of other chemicals with S9 prepared from PCB-pretreated rats. 10 carcinogens (e.g. 7, 12-dimethylbenz [a] anthracene, benzo-[a] pyrene, quinoline, etc.) out of 16 induced aberrations when they were treated with S9 mix, whereas the remaining 6 carcinogens (e.g., 3-methylcholanthrene, 4-o-tolylazo-o-toluidine, etc.) induced few or no aberrations even after activation. Two insecticides, allethrin and diazinon, were strongly positive at relatively low doses only when they were activated with the S9 mix. Medical drugs, such as ethenzamide, methyl p-hydroxybenzoate and nitrofurazone, and a food additive, sodium hypchlorite, were positive on activation. Chemicals used for industry, such as styrene monomer and tris-dichloropropylphosphate, were also positive in our activation system. © 1979.
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页码:277 / 290
页数:14
相关论文
共 25 条
[1]  
ABBONDANDOLO A, 1977, MUTAT RES, V46, P365, DOI 10.1016/0165-1161(77)90013-9
[2]   METHODS FOR DETECTING CARCINOGENS AND MUTAGENS WITH SALMONELLA-MAMMALIAN-MICROSOME MUTAGENICITY TEST [J].
AMES, BN ;
MCCANN, J ;
YAMASAKI, E .
MUTATION RESEARCH, 1975, 31 (06) :347-363
[3]  
BARTSCH H, 1975, CANCER RES, V35, P644
[4]   INDUCTION OF SISTER CHROMATID EXCHANGES BY CYCLOPHOSPHAMIDE IN PRESENCE OF DIFFERENTLY INDUCED MICROSOMAL FRACTIONS OF RAT-LIVER [J].
DERAAT, WK .
CHEMICO-BIOLOGICAL INTERACTIONS, 1977, 19 (01) :125-131
[5]  
GLETTEN F, 1975, MUTAT RES, V28, P113
[6]   RELATIONSHIP BETWEEN EXPERIMENTAL RESULTS IN MAMMALS AND MAN .1. CYTOGENETIC ANALYSIS OF BONE-MARROW INJURY INDUCED BY A SINGLE DOSE OF CYCLOPHOSPHAMIDE [J].
GOETZ, P ;
SRAM, RJ ;
DOHNALOVA, J .
MUTATION RESEARCH, 1975, 31 (04) :247-254
[7]   CHROMOSOME-ABERRATIONS AND DOMINANT LETHALITY OF MOUSE EMBRYOS AFTER PATERNAL TREATMENT WITH TRIETHYLENEMELAMINE [J].
HITOTSUMACHI, S ;
KIKUCHI, Y .
MUTATION RESEARCH, 1977, 42 (01) :117-124
[8]   CHROMOSOME TESTS WITH 134 COMPOUNDS ON CHINESE-HAMSTER CELLS INVITRO - SCREENING FOR CHEMICAL CARCINOGENS [J].
ISHIDATE, M ;
ODASHIMA, S .
MUTATION RESEARCH, 1977, 48 (3-4) :337-353
[9]   ROOT TIPS OF VICIA-FABA FOR STUDY OF INDUCTION OF CHROMOSOMAL-ABERRATIONS [J].
KIHLMAN, BA .
MUTATION RESEARCH, 1975, 31 (06) :401-412
[10]   CYTOTOXICITY OF HAIR COLORANT CONSTITUENTS - CHROMOSOME DAMAGE INDUCED BY 2 NITROPHENYLENEDIAMINES IN CULTURED CHINESE-HAMSTER CELLS [J].
KIRKLAND, DJ ;
VENITT, S .
MUTATION RESEARCH, 1976, 40 (01) :47-55