CHARACTERIZATION OF A PROGESTIN-BINDING MACROMOLECULE IN THE AMPHIBIA OOCYTE CYTOSOL

被引：14

作者：

KALIMI, M ^{[1
]}

ZIEGLER, D ^{[1
]}

MORRILL, GA ^{[1
]}

机构：

[1] YESHIVA UNIV,ALBERT EINSTEIN COLL MED,DEPT PHYSIOL,BRONX,NY 10461

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 86卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91750-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A [3H]-progestin-binding macromolecule has been isolated from R. pipiens oocyte cytosol and characterized using binding assays, gel filtration, DEAE-cellulose chromatography and ultracentrifugal techniques. Macromolecules present in the prophase oocyte cytosol have a high affinity and specificity for the synthetic progestin R5020 and the intact oocyte will concentrate both R5020 and progesterone 20-40 fold from the medium. This may be the first published case of a cytosol steroid binding macromolecule in a cell system in which the steroid appears to act at an extranuclear level. © 1979.

引用

页码：560 / 567

页数：8

共 19 条

[1]

BEATO M, 1972, J BIOL CHEM, V249, P5328

[2] EFFECTS OF ACTINOMYCIN PUROMYCIN AND CYCLOHEXIMIDE UPON MATURATION OF AMPHIBIAN OVOCYTES [J].

BRACHET, J .

EXPERIMENTAL CELL RESEARCH, 1967, 48 (01) :233-&

[3] BIOCHEMICAL CHANGES DURING PROGESTERONE-INDUCED MATURATION IN XENOPUS-LAEVIS OOCYTES [J].

BRACHET, J ;

BALTUS, E ;

DESCHUTT.A ;

HANOCQ, F ;

HANOCQQU.J ;

HUBERT, E ;

IACOBELLI, S ;

STEINERT, G .

MOLECULAR AND CELLULAR BIOCHEMISTRY, 1974, 3 (03) :189-205

[4]

DETTLAFF TA, 1966, J EMBRYOL EXP MORPH, V16, P183

[5] RELATIVE ROLES OF PITUITARY FOLLICLE CELLS AND PROGESTERONE IN INDUCTION OF OOCYTE MATURATION IN RANA PIPIENS [J].

MASUI, Y .

JOURNAL OF EXPERIMENTAL ZOOLOGY, 1967, 166 (03) :365-&

[6] ESTRADIOL RECEPTOR OF CALF UTERUS - INTERACTION WITH HEPARIN-AGAROSE AND PURIFICATION [J].

MOLINARI, AM ;

MEDICI, N ;

MONCHARMONT, B ;

PUCA, GA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (11) :4886-4890

[7] RNA AND PROTEIN-SYNTHESIS DURING PROGESTERONE-INDUCED GERMINAL VESICLE BREAKDOWN IN R-PIPIENS OVARIAN TISSUE [J].

MORRILL, GA ;

SCHATZ, F ;

ZABRENETZKY, VS .

DIFFERENTIATION, 1975, 4 (03) :143-152

[8] FEMALE STEROID-HORMONES AND TARGET-CELL NUCLEI [J].

OMALLEY, BW ;

MEANS, AR .

SCIENCE, 1974, 183 (4125) :610-620

[9] PROGESTERONE RECEPTOR ASSOCIATED WITH MELANOSOME FRACTION OF PLEURODELES-WALTLII OOCYTES (URODELA AMPHIBIAN) [J].

OZON, R ;

BELLE, R .

BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 320 (03) :588-593

[10] BINDING OF PROGESTERONE AND R-5020, A HIGHLY POTENT PROGESTIN, TO HUMAN ENDOMETRIUM AND MYOMETRIUM [J].

PHILIBERT, D ;

RAYNAUD, JP .

CONTRACEPTION, 1974, 10 (05) :457-466

← 1 2 →