SINDBIS VIRUS NSP1 FUNCTIONS IN NEGATIVE-STRAND RNA-SYNTHESIS

被引：73

作者：

WANG, YF ^{[1
]}

SAWICKI, SG ^{[1
]}

SAWICKI, DL ^{[1
]}

机构：

[1] MED COLL OHIO,DEPT MICROBIOL,POB 10008,TOLEDO,OH 43699

来源：

JOURNAL OF VIROLOGY | 1991年 / 65卷 / 02期

关键词：

D O I：

10.1128/JVI.65.2.985-988.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A mutation at nucleotide 1101 of Sindbis virus ts11 nsP1 caused temperature-sensitive negative-strand synthesis and suppressed the 24R phenotype, which is caused by a mutation in nsP4. Nonstructural proteins synthesized and accumulated by ts11 at 40-degrees-C did not cause the reactivation of negative-strand synthesis upon return to 30-degrees-C and did not prevent the formation of new replication complexes at 30-degrees-C.

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页码：985 / 988

页数：4

相关论文

共 16 条

[1] CLEAVAGE-SITE PREFERENCES OF SINDBIS VIRUS POLYPROTEINS CONTAINING THE NONSTRUCTURAL PROTEINASE - EVIDENCE FOR TEMPORAL REGULATION OF POLYPROTEIN PROCESSING INVIVO [J].

DEGROOT, RJ ;

HARDY, WR ;

SHIRAKO, Y ;

STRAUSS, JH .

EMBO JOURNAL, 1990, 9 (08) :2631-2638

[2] EVIDENCE THAT SINDBIS VIRUS NSP2 IS AN AUTOPROTEASE WHICH PROCESSES THE VIRUS NONSTRUCTURAL POLYPROTEIN [J].

DING, MX ;

SCHLESINGER, MJ .

VIROLOGY, 1989, 171 (01) :280-284

[3] MAPPING OF RNA- TEMPERATURE-SENSITIVE MUTANTS OF SINDBIS VIRUS - ASSIGNMENT OF COMPLEMENTATION GROUP-A, GROUP-B, AND GROUP-G TO NONSTRUCTURAL PROTEINS [J].

HAHN, YS ;

STRAUSS, EG ;

STRAUSS, JH .

JOURNAL OF VIROLOGY, 1989, 63 (07) :3142-3150

[4] PROCESSING THE NONSTRUCTURAL POLYPROTEINS OF SINDBIS VIRUS - NONSTRUCTURAL PROTEINASE IS IN THE C-TERMINAL HALF OF NSP2 AND FUNCTIONS BOTH IN CIS AND IN TRANS [J].

HARDY, WR ;

STRAUSS, JH .

JOURNAL OF VIROLOGY, 1989, 63 (11) :4653-4664

[5]

HARDY WR, 1990, VIROLOGY, V177, P199

[6] PROCESSING THE NONSTRUCTURAL POLYPROTEINS OF SINDBIS VIRUS - STUDY OF THE KINETICS INVIVO BY USING MONOSPECIFIC ANTIBODIES [J].

HARDY, WR ;

STRAUSS, JH .

JOURNAL OF VIROLOGY, 1988, 62 (03) :998-1007

[7] DELETION MAPPING OF SINDBIS VIRUS DI RNAS DERIVED FROM CDNAS DEFINES THE SEQUENCES ESSENTIAL FOR REPLICATION AND PACKAGING [J].

LEVIS, R ;

WEISS, BG ;

TSIANG, M ;

HUANG, H ;

SCHLESINGER, S .

CELL, 1986, 44 (01) :137-145

[8] PRODUCTION OF INFECTIOUS RNA TRANSCRIPTS FROM SINDBIS VIRUS CDNA CLONES - MAPPING OF LETHAL MUTATIONS, RESCUE OF A TEMPERATURE-SENSITIVE MARKER, AND INVITRO MUTAGENESIS TO GENERATE DEFINED MUTANTS [J].

RICE, CM ;

LEVIS, R ;

STRAUSS, JH ;

HUANG, HV .

JOURNAL OF VIROLOGY, 1987, 61 (12) :3809-3819

[9] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[10] TEMPERATURE SENSITIVE SHUT-OFF OF ALPHAVIRUS MINUS STRAND RNA-SYNTHESIS MAPS TO A NONSTRUCTURAL PROTEIN, NSP4 [J].

SAWICKI, DL ;

BARKHIMER, DB ;

SAWICKI, SG ;

RICE, CM ;

SCHLESINGER, S .

VIROLOGY, 1990, 174 (01) :43-52