PRIMARY STRUCTURE OF 2 FORMS OF GONADOTROPIN-RELEASING-HORMONE FROM BRAINS OF THE AMERICAN ALLIGATOR (ALLIGATOR-MISSISSIPPIENSIS)

被引：45

作者：

LOVEJOY, DA

FISCHER, WH

PARKER, DB

MCRORY, JE

PARK, M

LANCE, V

SWANSON, P

RIVIER, JE

SHERWOOD, NM

机构：

[1] SALK INST BIOL STUDIES,PEPTIDE BIOL LABS,LA JOLLA,CA 92037

[2] UNIV WASHINGTON,SCH FISHERIES,SEATTLE,WA 98195

[3] ZOOL SOC SAN DIEGO,SAN DIEGO,CA 92112

来源：

REGULATORY PEPTIDES | 1991年 / 33卷 / 02期

关键词：

REPRODUCTION; GNRH; REPTILE; ALLIGATOR BRAIN; AMINO ACID SEQUENCE; BRAIN PEPTIDE; HPLC;

D O I：

10.1016/0167-0115(91)90206-V

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Two forms of gonadotropin-releasing hormone (GnRH) have been purified from brains of the American alligator, Alligator mississippiensis, using reverse-phase high-pressure liquid chromatography (HPLC). The concentration of total GnRH was 8.8 ng/g of frozen brain tissue or 21.1 ng per brain. The amino acid sequence of each form of GnRH was determined using automated Edman degradation. The presence of the N-terminal pGlu residue was established by digestion studies with bovine pyroglutamyl aminopeptidase and coelution with synthetic forms of the native peptide. The primary structure of alligator GnRH I is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Gln-Pro-Gly-NH2 and alligator GnRH II is pGlu-His-Trp-Ser-His-Gly-Trp-Tyr-Pro-Gly-NH2.

引用

页码：105 / 116

页数：12

共 25 条

[1] IDENTIFICATION OF A MAMMALIAN GLUTAMINYL CYCLASE CONVERTING GLUTAMINYL INTO PYROGLUTAMYL PEPTIDES [J].