A NOVEL FORMAT FOR A RAPID SANDWICH EIA AND ITS APPLICATION TO THE IDENTIFICATION OF SNAKE-VENOMS

被引：26

作者：

COX, JC

MOISIDIS, AV

SHEPHERD, JM

DRANE, DP

JONES, SL

机构：

[1] Immunodiagnostics Section, Research and Development Division, Commonwealth Serum Laboratories Limited, Parkville

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1992年 / 146卷 / 02期

关键词：

NOVEL FIELD ASSAY; ENZYME IMMUNOASSAY; SNAKE VENOM IDENTIFICATION;

D O I：

10.1016/0022-1759(92)90230-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid sandwich enzyme immunoassay format is described where conjugate is lyophilised within the well in which the test reaction will occur. The format is straight forward to manufacture, has a prolonged shelf life, and eliminates one incubation and wash step from the usual test procedure. The technology has been applied to the development of a rapid assay for the identification of snake venom in clinical specimens. The resultant assay was specific and sensitive, provided rapid results and was appropriate for field use.

引用

页码：213 / 218

页数：6

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