ISOLATION OF A GENOMAL CLONE CONTAINING CHICKEN HISTONE GENES

被引：19

作者：

HARVEY, RP

WELLS, JRE

机构：

[1] Department of Biochemistry, University of Adelaide, Adelaide. SA

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 7卷 / 07期

关键词：

D O I：

10.1093/nar/7.7.1787

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used enriched chicken histone cDNA to select genomal clones from a chicken library. Because the cDNA probe also contained other sequences, a further screening of positive plaques with negative probes eliminated most non-histone gene clones. One 'positively-selected1 genomal clone, λCH-O1, hybridised with cloned sea-urchin histone genes and also detected histone genes in EcoRI-digested genomal sea-urchin DNA. Limited DNA sequencing of Haelll fragments identified two sequences within the coding region of chicken histone H2A. A third fragment predicted an amino acid sequence with strong homo-logy to an HI histone sequence. © 1979 Information Retrieval Limited.

引用

页码：1787 / 1798

页数：12

共 24 条

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