ENGINEERING OF ARTIFICIAL CELL-ADHESION PROTEINS BY GRAFTING THE ARG-GLY-ASP CELL ADHESIVE SIGNAL TO A CALPASTATIN SEGMENT

被引：8

作者：

HASHINO, K

SHIMOJO, T

KIMIZUKA, F

KATO, I

MAEDA, T

SEKIGUCHI, K

TITANI, K

机构：

[1] OSAKA MED CTR & RES INST MATERNAL & CHILD HLTH, IZUMI, OSAKA 59002, JAPAN

[2] FUJITA HLTH UNIV, SCH MED, INST COMPREHENS MED SCI, TOYOAKE, AICHI 47011, JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1992年 / 112卷 / 04期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123936

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new artificial cell adhesive protein was engineered by grafting the Arg-Gly-Asp (RGD) sequence, the minimal recognition signal of fibronectin for interaction with integrins, to a calpastatin segment by in vitro mutagenesis. The mutagenized protein showed cell adhesive activity in addition to calpain inhibitory activity. The RGD signal grafted to the calpastatin segment was recognized by the vitronectin receptor but not by the fibronectin receptor.

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页码：547 / 551

页数：5

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