CHARACTERIZATION OF A TRYPANOSOMA-CRUZI POLY(A)-BINDING PROTEIN AND ITS GENES

被引：39

作者：

BATISTA, JAN

TEIXEIRA, SMR

DONELSON, JE

KIRCHHOFF, LV

DESA, CM

机构：

[1] UNIV IOWA, DEPT BIOCHEM, IOWA CITY, IA 52242 USA

[2] UNIV BRASILIA, DEPT BIOL CELULAR, BR-70910900 BRASILIA, DF, BRAZIL

[3] UNIV IOWA, DEPT INTERNAL MED, IOWA CITY, IA 52242 USA

[4] DEPT VET AFFAIRS MED CTR, IOWA CITY, IA 52242 USA

[5] UNIV IOWA, HOWARD HUGHES MED INST, IOWA CITY, IA 52242 USA

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1994年 / 67卷 / 02期

关键词：

TRYPANOSOMA CRUZI; POLY(A)-BINDING PROTEIN; PROTEOLYTIC CLEAVAGE;

D O I：

10.1016/0166-6851(94)00133-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have characterized the biochemical properties of a 66-kDa poly(A)-binding protein (PABP1) in the protozoan Trypanosoma cruzi and isolated two classes of cDNAs encoding the protein. In concordance, Southern blots showed the presence of 2 gene copies. The two cDNA classes differ in the length of adenosine-rich segments in the 5' untranslated region and in point changes scattered throughout the sequence, but their 1650-bp open reading frames encode identical proteins. A single mRNA of 5.5 kb was detected, indicating that the noncoding regions are unusually long. Both the mRNA and the protein are constitutively expressed in all stages of T. cruzi life cycle. The biochemical properties and sequence comparisons show that the T. cruzi PABP1 is similar to the PABP1 of other eukaryotic organisms. These results indicate that PABP1 has been conserved throughout eukaryotic evolution.

引用

页码：301 / 312

页数：12

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