EFFECTS OF THAPSIGARGIN, AN INTRACELLULAR CA2+ PUMP INHIBITOR, ON INSULIN RELEASE BY RAT PANCREATIC B-CELL

被引：11

作者：

AIZAWA, T ^{[1
]}

YADA, T ^{[1
]}

ASANUMA, N ^{[1
]}

SATO, Y ^{[1
]}

ISHIHARA, F ^{[1
]}

HAMAKAWA, N ^{[1
]}

YAEKURA, K ^{[1
]}

HASHIZUME, K ^{[1
]}

机构：

[1] KAGOSHIMA UNIV,SCH MED,DEPT PHYSIOL,KAGOSHIMA,JAPAN

来源：

LIFE SCIENCES | 1995年 / 57卷 / 14期

关键词：

THAPSIGARGIN; PANCREATIC B-CELL; INSULIN RELEASE; INTRACELLULAR CA2+;

D O I：

10.1016/0024-3205(95)02095-Z

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

This is the first report as to the effects of thapsigargin (Tg), an inhibitor of intracellular Ca2+ pumps, on insulin release by pancreatic B-cells. Tg does not alter basal insulin release by the isolated islets, with 3 mM glucose. However, it potentiates high glucose-induced insulin release: potentiation of the first phase response is dose-related in a concentration range of 1.3-40 mu M. In isolated B-cells, Tg causes a minimal rise in basal cytosolic free calcium concentration ([Ca2+](i)) and eliminates high glucose-induced initial lowering of [Ca2+](i). Tg does not alter glucose oxidation by the islets and the islet insulin content. An elimination of glucose-induced sequestration of Ca2+ into Tg-sensitive intracellular pool(s) is considered to be the cause of Tg potentiation of glucose effect on insulin release.

引用

页码：1375 / 1381

页数：7

共 23 条

[1] THYROTROPIN-RELEASING-HORMONE RAPIDLY STIMULATES A BIPHASIC SECRETION OF PROLACTIN AND GROWTH-HORMONE IN GH4C1 RAT PITUITARY-TUMOR CELLS [J].