SYNTHESIS AND INTRACELLULAR-LOCALIZATION OF CHICK ACID ALPHA-GLUCOSIDASE IN CHICK ERYTHROCYTE HUMAN FIBROBLAST HETEROKARYONS - A MODEL SYSTEM FOR THE STUDY OF LYSOSOMAL-ENZYME SYNTHESIS

被引：1

作者：

SIPS, HJ

REUSER, AJJ

VANDERVEER, E

机构：

来源：

EXPERIMENTAL CELL RESEARCH | 1986年 / 162卷 / 02期

关键词：

D O I：

10.1016/0014-4827(86)90360-5

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The synthesis and localization of chick acid .alpha.-glucosidase has been studied in chick erythrocyte-human fibroblast heterokaryons. Monospecific antibodies raised against purified chick liver acid .alpha.-glucosidase were used. It was found that the acid .alpha.-glucosidase in the heterokaryons is of chick origin, and is localized in the same lysosomes as the human lysosomal enzymes. It is concluded that chick erythrocyte-human fibroblast heterokaryons provide a useful model system for the study of lysosomal enzyme synthesis and routing.

引用

页码：555 / 561

页数：7

共 18 条

[1] ULTRASTRUCTURE OF CHICK ERYTHROCYTE NUCLEI UNDERGOING REACTIVATION IN HETEROKARYONS AND ENUCLEATED CELLS [J].