ENDOTHELIN-1 INDUCES INTRACELLULAR CALCIUM RISE AND INOSITOL 1,4,5-TRISPHOSPHATE FORMATION IN CULTURED RAT AND HUMAN GLIOMA-CELLS

被引：40

作者：

ZHANG, W

SAKAI, N

YAMADA, H

FU, T

NOZAWA, Y

机构：

[1] GIFU UNIV,SCH MED,DEPT BIOCHEM,TSUKASAMACHI 40,GIFU 500,JAPAN

[2] GIFU UNIV,SCH MED,DEPT NEUROSURG,GIFU 500,JAPAN

来源：

NEUROSCIENCE LETTERS | 1990年 / 112卷 / 2-3期

关键词：

Endothelin-1; Glioma cell; Inositol 1,4,5-trisphosphate; Intracellular calcium;

D O I：

10.1016/0304-3940(90)90203-L

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The effects of endothelin-1 (ET) on the signal transduction in rat and human glioma cell line cells have been investigated. ET was found to initiate the increase of intracellular calcium ([Ca2+]i) levels in both C6 and A-172 cells, which was concurrent with the formation of inositol 1,4,5-trisphosphate (IP3(1,4,5)). In the presence of [ethylenebis(oxyethylenenitrilo)]tetraacetic acid (EGTA) in the incubation media, the duration of the intracellular calcium response was reduced, indicating that the ET-induced increase of intracellular calcium in glioma cells may be mediated by a dual mechanism, intracellular calcium mobilization and influx of extracellular calcium. These results suggest that ET may also act as a neuropeptide in the central nervous system. © 1990.

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页码：199 / 204

页数：6

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