QUANTITATIVE-DETERMINATION OF HEMOGLOBIN AND CYTOCHEMICAL STAINING FOR PEROXIDASE USING 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE, A SAFE SUBSTITUTE FOR BENZIDINE

被引：75

作者：

LIEM, HH

CARDENAS, F

TAVASSOLI, M

POHFITZPATRICK, MB

MULLEREBERHARD, U

机构：

[1] SCRIPPS CLIN & RES FDN,DEPT HEMATOL,LA JOLLA,CA 92037

[2] COLUMBIA UNIV COLL PHYS & SURG,DEPT DERMATOL,NEW YORK,NY 10032

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 98卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90157-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The quantitative determination of hemoglobin employing 3,3′,5,5′-tetramethylbenzidine dihydrochloride (TMB-d) is described. This agent can also be used for staining myeloperoxidase-containing granules in granulocytes. TMB-d is at least as sensitive a reagent as benzidine but, unlike benzidine and diaminobenzidine, it is not a carcinogen. © 1979.

引用

页码：388 / 393

页数：6

共 14 条

[1]

AURAMEAS S, 1970, INT REV CYTOL, V27, P349

[2]

Clayson D.B., 1976, CHEM CARCINOGENS, P366

[3] A MODIFICATION OF THE BENZIDINE METHOD FOR MEASUREMENT OF HEMOGLOBIN IN PLASMA AND URINE [J].