RAPID QUANTITATION OF INDIVIDUAL RNA SPECIES IN A COMPLEX POPULATION

被引：21

作者：

BIESSMANN, H ^{[1
]}

CRAIG, EA ^{[1
]}

MCCARTHY, BJ ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,DEPT BIOCHEM & BIOPHYS,SAN FRANCISCO,CA 94143

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 7卷 / 04期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1093/nar/7.4.981

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Many investigations require quantitation of one or more individual RNA species in complex populations. Existing methods are tedious when multiple samples are to be assayed. A method is presented which allows rapid and accurate quantitation of many species of RNA simultaneously. Recombinant plasmids containing cDNA insorts are electrophoresed in agarose and blotted to nitrocellulose. After hybridization with labeled RNA and autoradiography, bands are quantitated by scanning. The results were calibrated by solution hybridization. The approach has been validated through the use of plasmids containing inserts of Drosophila cDNA and RAN of cultured cells. © 1979 Information Retrieval Limited.

引用

页码：981 / 996

页数：16

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