CORRELATION BETWEEN THE DNA SUPERCOILING AND THE INITIATION OF TRANSCRIPTION BY ESCHERICHIA-COLI RNA-POLYMERASE INVITRO - ROLE OF THE SEQUENCES UPSTREAM OF THE PROMOTER REGION

被引：110

作者：

MISHRA, RK ^{[1
]}

GOPAL, V ^{[1
]}

CHATTERJI, D ^{[1
]}

机构：

[1] CTR CELLULAR & MOLEC BIOL,UPPAL RD,HYDERABAD 500007,ANDHRA PRADESH,INDIA

来源：

FEBS LETTERS | 1990年 / 260卷 / 02期

关键词：

(E. coli); Abortive initiation; RNA polymerase; Supercoiling; T7; promoter; Upstream sequence;

D O I：

10.1016/0014-5793(90)80121-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Binding of Escherichia coli RNA polymerase and the abortive initiation of transcription at the A2 promoter ofbacteriophage T7, separately cloned in pBR322, was found to be strongly dependent on the degree of supercoiling of the plasmid. Supercoiling does not seem to play any role in the initiation of transcription at the T7A1 promoter under identical conditions. Plasmid containing T7A2 promoter was found to be less amenable to S1 nuclease in comparison to that having T7A1. Sequence comparison reveals a high G/C content upstream to the -35 region of T7A2 which by extra duplex stability probably renders the initiation of transcription more dependent on the state of supercoiling of the template. © 1990.

引用

页码：273 / 276

页数：4

共 21 条

[1] USE OF SITE-SPECIFIC RECOMBINATION AS A PROBE OF DNA-STRUCTURE AND METABOLISM INVIVO [J].

BLISKA, JB ;

COZZARELLI, NR .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 194 (02) :205-218

[2] SUPERCOILING RESPONSE OF THE LAC PS PROMOTER INVITRO [J].

BOROWIEC, JA ;

GRALLA, JD .

JOURNAL OF MOLECULAR BIOLOGY, 1985, 184 (04) :587-598

[3] ACTIVATION AND INHIBITION OF TRANSCRIPTION BY SUPERCOILING [J].

BRAHMS, JG ;

DARGOUGE, O ;

BRAHMS, S ;

OHARA, Y ;

VAGNER, V .

JOURNAL OF MOLECULAR BIOLOGY, 1985, 181 (04) :455-465

[4] NEGATIVE SUPERCOILING INDUCES SPONTANEOUS UNWINDING OF A BACTERIAL PROMOTER [J].

DREW, HR ;

WEEKS, JR ;

TRAVERS, AA .

EMBO JOURNAL, 1985, 4 (04) :1025-1032

[5] BIOLOGY OF BACTERIAL DEOXYRIBONUCLEIC-ACID TOPOISOMERASES [J].

DRLICA, K .

MICROBIOLOGICAL REVIEWS, 1984, 48 (04) :273-289

[6] COMPLETE NUCLEOTIDE-SEQUENCE OF BACTERIOPHAGE-T7 DNA AND THE LOCATIONS OF T7 GENETIC ELEMENTS [J].

DUNN, JJ ;

STUDIER, FW .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) :477-535

[7] THE USE OF GEL-ELECTROPHORESIS TO DETECT AND STUDY NUCLEIC-ACID PROTEIN INTERACTIONS [J].

GARNER, MM ;

REVZIN, A .

TRENDS IN BIOCHEMICAL SCIENCES, 1986, 11 (10) :395-396

[8] DNA TOPOISOMERASES [J].

GELLERT, M .

ANNUAL REVIEW OF BIOCHEMISTRY, 1981, 50 :879-910

[9]

Johnston D. E., 1976, RNA POLYMERASE, P413

[10] DETERMINATION OF NUMBER OF SUPERHELICAL TURNS IN SIMIAN VIRUS 40 DNA BY GEL-ELECTROPHORESIS [J].

KELLER, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (12) :4876-4880

← 1 2 3 →