NOT CA2+ BUT CAMP IS THE 2ND MESSENGER FOR MORPHOLOGICAL-CHANGES IN RAT MEGAKARYOCYTE

被引：5

作者：

UNEYAMA, C ^{[1
]}

IMAZAWA, T ^{[1
]}

UNEYAMA, H ^{[1
]}

AKAIKE, N ^{[1
]}

KAWANISHI, T ^{[1
]}

TAKAHASHI, M ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC MED,DEPT BIOPLAST,FUKUOKA 812,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 211卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.1808

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ATP and thrombin both induced Ca2+ mobilization from intracellular Ca2+ store site of megakaryocyte, the progenitor cell of platelet (Uneyama C., Uneyama H. and Akaike N. (1993) J. Physiol. (Lond.), 470,73-749), Since in platelet, thrombin is known as a strong agonist and ADP is known as a weak agonist, we further investigated the effect of these agonists on megakaryocyte. Thrombin induced Ca2+ mobilization, 5-hydroxy tryptamine (5-HT) release and aggregatory morphological changes in megakaryocyte, but ATP induced only Ca2+ mobilization. Thrombin-induced 5-HT release was inhibited by adenylate cyclase-activating drugs, and the morphological changes could be induced by H-8, an inhibitor of cAMP-dependent protein kinase. These results suggest that the Ca2+ mobilization is not sufficient to induce morphological changes, and the signal to cause morphological changes in megakaryocyte may be cAMP. (C) 1995 Academic Press, Inc.

引用

页码：282 / 288

页数：7

共 21 条

[1]

Radley J.M., Scurfield G., Blood, 56, pp. 996-999, (1980)

[2]

Fedorko M.E., Laboratory Investigation, 36, pp. 321-328, (1977)

[3]

Fedorko M.E., Lab. Invest, 36, pp. 310-320, (1977)

[4]

Leven R.M., Nachmias V.T., J. Cell Biol, 92, pp. 313-323, (1982)

[5]

Miller J.L., Blood, 61, pp. 967-972, (1983)

[6]

Schick B.P., Schick P.K., Chase P.R., Biochimica Et Biophysica Acta, 663, pp. 239-248, (1981)

[7]

Schik B.P., Walsh C.J., Jenkins-West T., J. Biol. Chem, 263, pp. 1052-1062, (1988)

[8]

Zucker-Franklin D., Petursson S., J. Cell Biol, 99, pp. 390-402, (1984)

[9]

Horn R., Marty A., J. Gen. Physiol, 92, pp. 145-159, (1988)

[10]

Uneyama C., Uneyama H., Akaike N., J. Physiol, 470, pp. 731-749, (1993)

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