RESTRICTION END-CONVERTING VECTORS WITH TANDEM REPEATED MULTIPLE CLONING SITES

被引：5

作者：

DELAFUENTE, A ^{[1
]}

CISNEROS, E ^{[1
]}

TALAVERA, A ^{[1
]}

机构：

[1] CTR NACL BIOTECHNOL,E-28049 MADRID,SPAIN

来源：

GENE | 1994年 / 139卷 / 01期

关键词：

RECOMBINANT DNA; PLASMIDS; SUBCLONING; PUROMYCIN-N-ACETYLTRANSFERASE;

D O I：

10.1016/0378-1119(94)90527-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The construction, characterization and uses of four vectors with novel multiple cloning sites (MCS) are described. The MCS consist of a central zone with unique restriction sites, flanked by two zones in which four sites are repeated in a direct sense. These vectors allow the insertion of fragments with the same or different restriction ends and their rescue with a multiple combination of ends, identical or different. This system conserves the XGa1 blue-white selection characteristics of the pUC plasmids, as well as their use as sequencing vehicles.

引用

页码：83 / 86

页数：4

共 14 条

[1] PRECISE AND NEARLY-PRECISE EXCISION OF THE SYMMETRICAL INVERTED REPEATS OF TN5 - COMMON FEATURES OF RECA-INDEPENDENT DELETION EVENTS IN ESCHERICHIA-COLI [J].