ALDOSTERONE MODULATES BOTH THE NA/H ANTIPORT AND CL/HCO3 EXCHANGER IN CULTURED NEONATAL RAT CARDIAC-CELLS

被引:38
作者
KORICHNEVA, I
PUCEAT, M
MILLANVOYEVANBRUSSEL, E
GERAUD, G
VASSORT, G
机构
[1] FAC MED NECKER ENFANTS MALAD, PHARMACOL REGULAT CARDIOVASC LAB, CNRS, URA 1482, F-75730 PARIS 15, FRANCE
[2] INST JACQUES MONOD, CNRS, UMR 9922, F-75251 PARIS 05, FRANCE
关键词
NEONATAL RAT CARDIOMYOCYTES; DEVELOPMENT; PH(I); IONIC TRANSPORTERS; MINERALOCORTICOID;
D O I
10.1006/jmcc.1995.0239
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Mineralocorticoid hormones regulate many physiological functions in the cardiovascular system. Although high affinity binding sites for aldosterone have been found in myocardium, aldosterone effects on pH(i) regulatory systems in cardiac cells have not been described. We have addressed this issue by using microspectrofluorimetric monitoring of intracellular pH in developing neonatal rat cardiomyocytes cultured for 2 weeks. Developmental changes in cell morphology were controlled by anti-myosin light chain antibody staining of the sarcomeric units using confocal laser scanning microscopy. The data obtained demonstrate that from early stages of the development, pH(i) in neonatal cardiac cells is regulated by three ion transporting mechanisms, namely, Na/H antiport, Na- and HCO3-dependent transporter and Cl/HCO3 exchanger. A 24-h treatment of the cells with aldosterone increases the activity of the Cl/HCO3 exchanger at day 6 of cell culture while the Na/H antiport activity is enhanced in the cells treated with the hormone at days 9 and 13 of culture. Thus, by affecting the activity of ionic transporters, aldosterone modulates acid-base balance in cardiac cells. (C) 1995 Academic Press Limited
引用
收藏
页码:2521 / 2528
页数:8
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