INTERACTIVE REGULATION OF SIGNALING PATHWAYS IN BONE-CELLS - POSSIBLE MODULATION OF PGE2-STIMULATED ADENYLYL CYCLASE ACTIVITY BY PROTEIN KINASE-C

被引：9

作者：

DOBSON, PRM ^{[1
]}

BROWN, BL ^{[1
]}

MICHELANGELI, VP ^{[1
]}

SHORT, AD ^{[1
]}

MOSELEY, JM ^{[1
]}

RUSSELL, RGG ^{[1
]}

MARTIN, TJ ^{[1
]}

机构：

[1] ST VINCENTS HOSP,ST VINCENTS INST MED RES,MELBOURNE,VIC,AUSTRALIA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1052卷 / 02期

基金：

英国惠康基金;

关键词：

cAMP interactions; Prostaglandin E[!sub]2[!/sub; Protein kinase C; Vasoactive intestinal polypeptide;

D O I：

10.1016/0167-4889(90)90228-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have reported previously that tumour-promoting phorbol esters modulate both basal and vasoactive intestinal polypeptide (VIP)-stimulated adenylyl cyclase activity in GH3 (an established pituitary cell line). Here, we probe the receptor and cell specificity of this response. Experiments were performed in the presence of isobutylmethylxanthine. Unlike the response in GH3 cells, the tumour-promoting phorbol ester (tetradecanoyl phorbol acetate (TPA)) did not affect either basal adenylyl cyclase activity nor VIP-stimulated activity in the rat osteosarcoma subclones UMR 106-01 and UMR 106-06. In addition, the cyclase responses to parathyroid hormone (PTH), and, in the case of UMR 106-06, to calcitonin were unaffected by tumour-promoting phorbol ester. However, prostaglandin E2-stimulated cyclase activity in both of these subclones was attenuated in a dose-dependent manner. © 1990.

引用

页码：323 / 326

页数：4

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