EFFECTS OF ORGANIC AND INORGANIC SELENIUM-COMPOUNDS ON RAT MAMMARY-TUMOR CELLS

被引：67

作者：

RONAI, Z ^{[1
]}

TILLOTSON, JK ^{[1
]}

TRAGANOS, F ^{[1
]}

DARZYNKIEWICZ, Z ^{[1
]}

CONAWAY, CC ^{[1
]}

UPADHYAYA, P ^{[1
]}

ELBAYOUMY, K ^{[1
]}

机构：

[1] NEW YORK MED COLL, CTR CANC, ELMSFORD, NY 10595 USA

来源：

INTERNATIONAL JOURNAL OF CANCER | 1995年 / 63卷 / 03期

关键词：

D O I：

10.1002/ijc.2910630322

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To explore cellular effects of potent organoselenium chemopreventive agents we have used a rat mammary tumor cell line. We demonstrate that 1,4-phenylenebis(methylene) selenocyanate (p-XSC) at a dose of 5 mu M is a more potent inhibitor of DNA, RNA and protein synthesis as well as of mitochondrial transmembrane potential than its chemopreventive counterparts benzyl selenocyanate (BSC) and sodium selenite. These differences were also reflected in reduced growth rate by 24 and 48 hr. Cell-cycle and cell-morphology analysis revealed that higher doses of p-XSC (10 mu M) caused DNA fragmentation which was accompanied with partial loss of nuclear stainability, whereas BSC caused a noticeable change in cell-cycle distribution and extensive micronucleation. Overall, our results point to cellular targets of selenium compounds which may mediate their chemopreventive activities in mammary tissues. (C) 1995 Wiley-Liss, Inc.

引用

页码：428 / 434

页数：7

共 27 条

[1] ACTIVATION OF PROGRAMMED CELL-DEATH (APOPTOSIS) BY CISPLATIN, OTHER ANTICANCER DRUGS, TOXINS AND HYPERTHERMIA [J].