A PERIODIC NETWORK OF G-PROTEIN BETA-GAMMA SUBUNIT COEXISTING WITH CYTOKERATIN FILAMENT IN STARFISH OOCYTES

被引：16

作者：

CHIBA, K

LONGO, FJ

KONTANI, K

KATADA, T

HOSHI, M

机构：

[1] UNIV IOWA,DEPT ANAT,IOWA CITY,IA 52242

[2] UNIV TOKYO,FAC PHARMACEUT SCI,TOKYO,JAPAN

来源：

DEVELOPMENTAL BIOLOGY | 1995年 / 169卷 / 02期

关键词：

D O I：

10.1006/dbio.1995.1157

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Heterotrimeric G proteins are membrane-bound and carry signals from activated receptors on plasma membranes to cytoplasmic effector enzymes and channels. In starfish oocytes, the beta gamma subunit of G protein mediates 1-methyladenine stimulation of oocyte maturation. In order to investigate the localization of beta gamma subunits in starfish oocytes during oocyte maturation, we raised a monoclonal antibody against the beta subunit. By immunofluorescence microscopy using the antibody, immature oocytes show a network of fibers in the cytoplasm. The staining of fibers is beaded with a periodicity of 0.7 mu m. The same staining pattern is obtained by anti-gamma subunit antibody. In addition, the fibers are stained by anti-cytokeratin antibody. These results indicate that the G protein gamma subunit coexists with cytokeratin filaments in starfish oocytes. Stimulation of oocyte maturation by 1-methyladenine causes the beta gamma subunit to be disassembled. (C) 1995 Academic Press,Inc.

引用

页码：415 / 420

页数：6

共 24 条

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