RESTRICTION ENDONUCLEASE CLEAVAGE MAP OF BACTERIOPHAGE-P2

被引：22

作者：

WESTOO, A

LJUNGQUIST, E

机构：

[1] Microbial Genetics Laboratory, Karolinska Institutet, Stockholm

来源：

MOLECULAR & GENERAL GENETICS | 1979年 / 171卷 / 01期

关键词：

D O I：

10.1007/BF00274019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A restriction endonuclease cleavage map of phage P2 was constructed. The enzymes used and, within parenthesis, the number of their cleavage sites on the P2 lg cc DNA molecule were: AvaI(3), BalI(1), BamI(3), BglII(3), HaeIII (more than 40; only three were mapped), HindIII(0), HpaI(10), KpnI(3), PstI(3), SalI(2) and SmaI(1). The EcoRI cleavage sites (3), as determined earlier, were used as reference points for this study. The DNAs of a variety of P2 mutants carrying chromosomal aberrations (del1, del2, del3, del6, vir22, vir37(2), vir79 and vir94) were also similarly examined. © 1979 Springer-Verlag.

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页码：91 / 102

页数：12

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