HOW TO APPROACH PREPARATIVE LIQUID-CHROMATOGRAPHY

被引：29

作者：

COQ, B

CRETIER, G

GONNET, C

ROCCA, JL

机构：

[1] E. R. A. 474, Laboratoire de Chimie Analytique III, Université Claude Bernard-Lyon 1, Villeurbanne, F-69621

来源：

CHROMATOGRAPHIA | 1979年 / 12卷 / 03期

关键词：

Chromatography; Liquid; Overloading; Preparative; Volume;

D O I：

10.1007/BF02314866

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This work shows the influence of the injection volume on peak broadening owing to the increase of the sample concentration; use of the maximum injection volume is advocated. Once the chromatographic system is defined, a method for calculating the dimensions of the preparative column is developed. Applications of this method are shown. © 1979 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

引用

收藏

页码：139 / 146

页数：8

相关论文

共 13 条

[1]

[Anonymous], 2021, AACR ANN M APR MAY

[2] TECHNIQUES FOR PACKING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC COLUMNS WITH POROUS MICROPARTICLES [J].

COQ, B ;

GONNET, C ;

ROCCA, JL .

JOURNAL OF CHROMATOGRAPHY, 1975, 106 (02) :249-262

[3]

COQ B, UNPUBLISHED

[4] PERFORMANCE OF LARGER DIAMETER COLUMNS FOR HIGH-SPEED LIQUID-SOLID CHROMATOGRAPHY [J].

DESTEFANO, JJ ;

BEACHELL, HC .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1972, 10 (11) :654-+

[5] SAMPLE LOADING AND EFFICIENCY IN ADSORPTION, PARTITION AND BONDED-PHASE HIGH-SPEED LIQUID-CHROMATOGRAPHY [J].

DONE, JN .

JOURNAL OF CHROMATOGRAPHY, 1976, 125 (01) :43-57

[6] INFLUENCE OF PARTICLE-SIZE (5-35MUM) OF SPHERICAL SILICA ON COLUMN EFFICIENCIES IN HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].

ENDELE, R ;

HALASZ, I ;

UNGER, K .

JOURNAL OF CHROMATOGRAPHY, 1974, 99 (NOV6) :377-393

[7] USE OF AN 18-MM ID COLUMN FOR ANALYTICAL-SCALE AND SEMI-PREPARATIVE-SCALE HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].

GODBILLE, E ;

DEVAUX, P .

JOURNAL OF CHROMATOGRAPHY, 1976, 122 (JUL7) :317-329

[8]

MAJORS RE, 1972, ANAL CHEM, V44, P122

[9] SOME ASPECTS OF PREPARATIVE-SCALE LIQUID-CHROMATOGRAPHY [J].

SCOTT, RPW ;

KUCERA, P .

JOURNAL OF CHROMATOGRAPHY, 1976, 119 (APR28) :467-482

[10] EXAMINATION OF SOME COMMERCIALLY AVAILABLE SILICA-GEL ADSORBENTS FOR USE IN LIQUID SOLID CHROMATOGRAPHY [J].

SCOTT, RPW ;

KUCERA, P .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1974, 12 (09) :473-485