DETERMINATION OF SOLUTION CONFORMATION OF DNA BACKBONE - APPLICATION OF HOMONUCLEAR (J-DELTA) SPECTROSCOPY

被引：15

作者：

HOSUR, RV ^{[1
]}

CHARY, KVR ^{[1
]}

SARAN, A ^{[1
]}

GOVIL, G ^{[1
]}

MILES, HT ^{[1
]}

机构：

[1] NIDDKD,MOLEC BIOL LAB,BETHESDA,MD 20892

来源：

BIOPOLYMERS | 1990年 / 29卷 / 6-7期

关键词：

D O I：

10.1002/bip.360290608

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Homonuclear two‐dimensional (J,δ) proton spectroscopy has been suggested as a method for the measurement of 1H‐31P coupling constants in oligonucleotides. The technique has been applied to a dinucleoside monophosphate G2′p5′C and a deoxydecanucleotide d(ACATCGATGT). PCILO energy calculations have been carried out to find minimum energy conformations with respect to the DNA backbone torsion angle ε, and these have been considered for the interpretation of the observed H3′‐31P coupling constants in oligonucleotides. Copyright © 1990 John Wiley & Sons, Inc.

引用

收藏

页码：953 / 959

页数：7

相关论文

共 28 条

[1] HOMONUCLEAR BROAD-BAND DECOUPLING AND 2-DIMENSIONAL J-RESOLVED NMR-SPECTROSCOPY [J].

AUE, WP ;

KARHAN, J ;

ERNST, RR .

JOURNAL OF CHEMICAL PHYSICS, 1976, 64 (10) :4226-4227

[2] SEQUENCE-SPECIFIC SOLUTION STRUCTURE OF D-GGTACGCGTACC [J].

CHARY, KVR ;

HOSUR, RV ;

GOVIL, G ;

CHEN, CQ ;

MILES, HT .

BIOCHEMISTRY, 1988, 27 (10) :3858-3867

[3] NOVEL SOLUTION CONFORMATION OF DNA OBSERVED IN D(GAATTCGAATTC) BY TWO-DIMENSIONAL NMR-SPECTROSCOPY [J].

CHARY, KVR ;

HOSUR, RV ;

GOVIL, G ;

TAN, ZK ;

MILES, HT .

BIOCHEMISTRY, 1987, 26 (05) :1315-1322

[4] THE STRUCTURE OF THE DOUBLE-STRANDED-RNA PENTAMER 5'(CACAG).5'(CUGUG) DETERMINED BY NUCLEAR OVERHAUSER ENHANCEMENT MEASUREMENTS - INTERPROTON DISTANCE DETERMINATION AND STRUCTURE REFINEMENT ON THE BASIS OF X-RAY COORDINATES [J].

CLORE, GM ;

GRONENBORN, AM ;

MCLAUGHLIN, LW .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 151 (01) :153-165

[5]

Ernst R. R., 1987, PRINCIPLES NUCL MAGN, V1st

[6] ASSIGNMENTS OF P-31 NMR RESONANCES IN OLIGODEOXYRIBONUCLEOTIDES - ORIGIN OF SEQUENCE-SPECIFIC VARIATIONS IN THE DEOXYRIBOSE PHOSPHATE BACKBONE CONFORMATION AND THE P-31 CHEMICAL-SHIFTS OF DOUBLE-HELICAL NUCLEIC-ACIDS [J].

GORENSTEIN, DG ;

SCHROEDER, SA ;

FU, JM ;

METZ, JT ;

ROONGTA, V ;

JONES, CR .

BIOCHEMISTRY, 1988, 27 (19) :7223-7237

[7]

GOVIL G, 1982, CONFORMATION BIOL MO

[8] CORRELATION OF CONNECTED TRANSITIONS BY TWO-DIMENSIONAL NMR-SPECTROSCOPY [J].

GRIESINGER, C ;

SORENSEN, OW ;

ERNST, RR .

JOURNAL OF CHEMICAL PHYSICS, 1986, 85 (12) :6837-6852

[9] EXTRAHELICAL ADENOSINE STACKS INTO RIGHT-HANDED DNA - SOLUTION CONFORMATION OF THE D(C-G-C-A-G-A-G-C-T-C-G-C-G) DUPLEX DEDUCED FROM DISTANCE GEOMETRY ANALYSIS OF NUCLEAR OVERHAUSER EFFECT SPECTRA [J].

HARE, D ;

SHAPIRO, L ;

PATEL, DJ .

BIOCHEMISTRY, 1986, 25 (23) :7456-7464

[10] WOBBLE DG.DT PAIRING IN RIGHT-HANDED DNA - SOLUTION CONFORMATION OF THE D(C-G-T-G-A-A-T-T-C-G-C-G) DUPLEX DEDUCED FROM DISTANCE GEOMETRY ANALYSIS OF NUCLEAR OVERHAUSER EFFECT SPECTRA [J].

HARE, D ;

SHAPIRO, L ;

PATEL, DJ .

BIOCHEMISTRY, 1986, 25 (23) :7445-7456