GENETIC AND BIOCHEMICAL-ANALYSIS OF GLUTATHIONE-DEFICIENT MUTANTS OF SACCHAROMYCES-CEREVISIAE

被引：29

作者：

KISTLER, M

MAIER, K

ECKARDTSCHUPP, F

机构：

[1] GESELL STRAHLEN & UMWELTFORSCH MBH,INST STRAHLENBIOL,W-8042 NEUHERBERG,GERMANY

[2] GESELL SCHWERIONENFORSCH GMBH,PROJEKT INHALAT,W-8042 NEUHERBERG,GERMANY

来源：

MUTAGENESIS | 1990年 / 5卷 / 01期

关键词：

D O I：

10.1093/mutage/5.1.39

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Five independently isolated glutathione-deficient (gsh-) mutants of Saccharomyces cerevisae with maximally 6% residual glutathione content have been analysed genetically. Complementation as well as tetrad analysis of the homo-and heterozygous diploids constructed by suitable crosses of the five mutants indicated that all isolates belong to one complementation group and hence represent different alleles of one gene, GSHL In order to determine the Gshl gene product an assay suitable for yeast was developed to determine the activity of γ-glutamyl-cysteine synthetase catalysing the first step of glutathione biosynthesis. All mutants are severely deficient in γ-glutamyl-cysteine synthetase (6.5% of the activity of the competent parental strain) which is in good accordance with the genetic data. © 1990 Oxford University Press.

引用

页码：39 / 44

页数：6

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