FSH STIMULATION OF GLUCOSAMINE-H-3-INCORPORATION INTO PROTEOGLYCANS BY PORCINE GRANULOSA-CELLS INVITRO

Porcine granulosa cells from small follicles were incubated in the presence of 1 μCi/ml3H-glucosamine and various hormones. Proteoglycans were digested with protease, and the mucopolysaccharides (MPS) were recovered. Most of the labeled MPS was found in secretory products. Maximum incorporation of3H-glucosamine into MPS occurred after incubation with FSH for 8-16 h. Cell concentrations of 0.5-4.0 x 106/ml proved to be ample. Highly purified porcine or human FSH gave consistent, significant stimulation by doses in the order of 100 pg/ml. Linear log-dose response curves occurred from 0.1 to 10.0 ng/ml, and maximal stimulation at 10 ng/ml was 22-fold above control. Preparations of hCG, hTSH and NIH-LH-S18 were not effective. Incubations with 1-10 mM dibutyryl cyclic AMP significantly increased incorporation into MPS, suggesting that effects of FSH may be mediated by cyclic AMP. © 1979 by The Endocrine Society.