TYROSINE-HYDROXYLASE AND POMC MESSENGER-RNA IN THE ARCUATE REGION ARE INCREASED BY CASTRATION AND HYPERPROLACTINEMIA

被引：40

作者：

SELMANOFF, M ^{[1
]}

SHU, C ^{[1
]}

HARTMAN, RD ^{[1
]}

BARRACLOUGH, CA ^{[1
]}

PETERSEN, SL ^{[1
]}

机构：

[1] UNIV MISSOURI,SCH MED,DEPT ANAT & NEUROBIOL,COLUMBIA,MO 65212

来源：

MOLECULAR BRAIN RESEARCH | 1991年 / 10卷 / 03期

关键词：

INSITU HYBRIDIZATION HISTOCHEMISTRY; TYROSINE HYDROXYLASE MESSENGER RNA; PROOPIOMELANOCORTIN MESSENGER RNA; HYPERPROLACTINEMIA; CASTRATION; ARCUATE REGION;

D O I：

10.1016/0169-328X(91)90072-6

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have examined the changes which occur in neuronal expression of tyrosine hydroxylase (TH) and proopiomelanocortin (POMC) mRNA in response to castration and hyperprolactinemia (HP) in male rats. Steady-state mRNA levels were determined by quantitative in situ hybridization histochemistry (ISHH) using S-35-labeled synthetic 48-base oligodeoxynucleotide probes. Castration produced a 27% increase in TH mRNA in the periventricular and arcuate nuclei. PRL-exposed rats exhibited a further 27% increase in the level of TH mRNA and a striking 48% increase in POMC mRNA in periarcuate region cell bodies. These results indicate that gonadal steroids and PRL are involved, either directly or indirectly, in regulating the biosynthesis of TH and POMC in the hypothalamus.

引用

页码：277 / 281

页数：5

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