BIOSYNTHESIS OF FERULIC ACID-ESTERS OF PLANT-CELL WALL POLYSACCHARIDES IN ENDOMEMBRANES FROM PARSLEY CELLS

被引：44

作者：

MEYER, K ^{[1
]}

KOHLER, A ^{[1
]}

KAUSS, H ^{[1
]}

机构：

[1] UNIV KAISERSLAUTERN,FACHBEREICH BIOL,POSTFACH 3049,W-6750 KAISERSLAUTERN,GERMANY

来源：

FEBS LETTERS | 1991年 / 290卷 / 1-2期

关键词：

PARSLEY SUSPENSION CELL; FERULIC ACID ESTER; CELL WALL POLYSACCHARIDE; GOLGI MEMBRANE; FERULOYL-COA;

D O I：

10.1016/0014-5793(91)81261-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A microsomal preparation from suspension-cultured parsley cells is able to transfer ferulic acid from the respective CoA thioester to endogenous acceptors. The reaction is not enhanced by digitonin but stimulated by Mg2+, Ca2+ and Co2+. Spermine can partly replace divalent ions. Solubility properties and degradation by polysaccharide hydrolases suggest that the products are polymeric cell wall carbohydrates. Sucrose density gradient centrifugation revealed that the most active vesicle fraction is distinct from plasma membranes but does also not peak with IDPase. It is suggested that a subfraction of the Golgi-apparatus is the source of enzyme and acceptors.

引用

页码：209 / 212

页数：4

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