CLONING AND NUCLEOTIDE SEQUENCING OF THE GENES, RPLU AND RPMA, FOR RIBOSOMAL-PROTEINS L21 AND L27 OF ESCHERICHIA-COLI

被引：11

作者：

JEONG, JH ^{[1
]}

KITAKAWA, M ^{[1
]}

ISONO, S ^{[1
]}

ISONO, K ^{[1
]}

机构：

[1] KOBE UNIV, FAC SCI, DEPT BIOL, KOBE 657, JAPAN

来源：

DNA SEQUENCE | 1993年 / 4卷 / 01期

关键词：

CRTE; ESCHERICHIA COLI; NUCLEOTIDE SEQUENCE; RIBOSOMAL PROTEIN GENES;

D O I：

10.3109/10425179309015624

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The rplU and rpmA genes that encode ribosomal proteins (r-proteins) L21 and L27 of Escherichia coli K-12 have been isolated from the ordered clone bank of this bacterium. They were found to be located at coordinates 3,351.7-3,352.3 kb on the physical map of E. coli. The nucleotide sequence of the cloned genes and their flanking regions indicated that the two r-protein genes compose an operon. Upstream of the two genes there is an open reading frame (ORF) in the opposite direction. The deduced polypeptide encoded by this ORF has a molecular weight of 35,215 and shows a significant degree of sequence similarity to the eznyme that is involved in the carotenoid biosynthesis and encoded by the crtE gene of carotenogenic bacteria and to prenyltransferases found in various organisms.

引用

页码：59 / 67

页数：9

共 20 条

[1]

ASHBY MN, 1990, J BIOL CHEM, V265, P13157

[2]

CARATTOLI A, 1991, J BIOL CHEM, V266, P5854

[3] PRIMARY STRUCTURE OF PROTEIN L27 FROM PEPTIDYL-TRANSFER-RNA BINDING-SITE OF ESCHERICHIA-COLI RIBOSOMES [J].