CLONING, SEQUENCING AND EXPRESSION OF CDNA-ENCODING AN INSECT V-ATPASE SUBUNIT-E

被引：27

作者：

GRAF, R

HARVEY, WR

WIECZOREK, H

机构：

[1] UNIV MUNICH,INST ZOOL,D-80021 MUNICH,GERMANY

[2] TEMPLE UNIV,DEPT BIOL,PHILADELPHIA,PA 19122

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1994年 / 1190卷 / 01期

关键词：

VACUOLAR H+-ATPASE; ATPASE; H+-; INSECT MIDGUT; CDNA CLONING; (MANDUCA-SEXTA);

D O I：

10.1016/0005-2736(94)90053-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This work presents the first invertebrate cDNA sequence encoding subunit E of a V-ATPase. It was cloned by immune-shotgun screening of a Manduca sexta (Insecta, Lepidoptera, Sphingidae) posterior larval midgut cDNA library. The amino acid sequence was 64% identical to that of the mammalian E-subunit and 34% to that of yeast. Southern and Northern blots suggested the existence of only one gene encoding the insect subunit E.

引用

页码：193 / 196

页数：4

共 18 条

[1] ANALYSIS OF THE GENE ENCODING A 16-KDA PROTEOLIPID SUBUNIT OF THE VACUOLAR H+-ATPASE FROM MANDUCA-SEXTA MIDGUT AND TUBULES [J].