DNAK/DNAJ SUPPLEMENTATION IMPROVES THE PERIPLASMIC PRODUCTION OF HUMAN GRANULOCYTE-COLONY-STIMULATING FACTOR IN ESCHERICHIA-COLI

被引：43

作者：

PEREZPEREZ, J ^{[1
]}

MARTINEZCAJA, C ^{[1
]}

BARBERO, JL ^{[1
]}

GUTIERREZ, J ^{[1
]}

机构：

[1] PHARM ANTIBOT FARMA SA,DEPT INVEST,E-28026 MADRID,SPAIN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 210卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.1691

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human granulocyte-colony stimulating factor (hG-CSF) can be efficiently synthesized in E. coli as a fusion to a quimeric signal peptide, but this fusion is only partially exported to the periplasm. We have studied the effects of supplementing the host content in chaperonins on the secretion of prehG-CSF in E. coli. We have found that the DnaK/DnaJ chaperonin system improves the secretion of this recombinant protein. This improvement correlates with an increase in the solubility of the precursor. Only a small fraction of the mature protein is released from the periplasm by an osmotic shock. (C) Academic Press, Inc.

引用

页码：524 / 529

页数：6

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