PROTEOLYTIC DENATURATION AND METHODS OF IMPROVING THE STABILITY OF GLUCOSE-ISOMERASE PREPARATIONS

被引：8

作者：

ADLER, D ^{[1
]}

LIM, H ^{[1
]}

COTTLE, D ^{[1
]}

EMERY, A ^{[1
]}

机构：

[1] PURDUE UNIV,SCH CHEM ENGN,W LAFAYETTE,IN 47907

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1979年 / 21卷 / 08期

关键词：

D O I：

10.1002/bit.260210804

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Evidence is provided in support of proteolytic denaturation of free and immobilized preparations of glucose isomerase from a Bacillus species. A number of methods to improve the stability with respect to proteolysis have been tested and their advantages as well as shortcomings are discussed. These methods include hollow‐fiber treatment, gel permeation, thermal treatment, and addition of protease inhibitors. The half‐life of the free and the cellulose acetate fiber‐entrapped preparations of glucose isomerase can be significantly improved. For example, the hollow‐fiber treatment can improve the half‐life by an order of magnitude. Copyright © 1979 John Wiley & Sons, Inc.

引用

页码：1345 / 1359

页数：15

共 14 条

[1]

ADLER D, 1976, THESIS PURDUE U

[2]

CHEN BJ, 1973, THESIS PURDUE U

[3]

DANNO G-I, 1970, Agricultural and Biological Chemistry, V34, P1795

[4]

DAVIS JC, 1974, CHEM ENG-NEW YORK, V81, P52

[5]

FOGARTY WM, 1974, PROCESS BIOCHEM, V9, P27

[6]

GARAVAGLIA A, 1974, THESIS PURDUE U

[7]

HAMILTON BK, 1974, IMMOBILIZED ENZYMES, P85

[8]

KOLARIK MJ, 1974, THESIS PURDUE U

[9]

KOLARIK MJ, 1974, IMMOBILIZED ENZYMES, P71

[10] ECONOMIC IMPLICATIONS OF PURIFICATION OF GLUCOSE ISOMERASE PRIOR TO IMMOBILIZATION [J].

LADISCH, MR ;

EMERY, A ;

RODWELL, VW .

INDUSTRIAL & ENGINEERING CHEMISTRY PROCESS DESIGN AND DEVELOPMENT, 1977, 16 (03) :309-313

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