HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF COBALAMINS

被引：26

作者：

FRENKEL, EP

KITCHENS, RL

PROUGH, R

机构：

[1] UNIV TEXAS,HLTH SCI CTR,SW MED SCH,DEPT BIOCHEM,DALLAS,TX 75235

[2] VET ADM HOSP,MED CTR,DALLAS,TX 75216

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 174卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0021-9673(00)86013-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Physiological cobalamins were separated by means of high-performance liquid chromatography (HPLC). Optimal conditions for elution of methylcobalamin, adenosylcobalamin, hydroxycobalamin and cyanocobalamin were determined. Excellent separation and resolution of these physiological cobalamins by HPLC were achieved. In addition, several cobalamin analogues were also studied and shown to be separable from the physiological forms. HPLC provides a rapid, sensitive, reproducible means of characterizing physiological cobalamins. © 1979.

引用

页码：393 / 400

页数：8

共 15 条

[1] COMPETITIVE INTRINSIC-FACTOR BINDING ASSAY TECHNIQUE FOR COBALAMINS IN NATURAL-WATERS [J].