IDENTIFICATION OF RECOVERIN-LIKE IMMUNOREACTIVITY IN MOUSE-BRAIN

被引:18
作者
TAKAMATSU, K [1 ]
UYEMURA, K [1 ]
机构
[1] KEIO UNIV,SCH MED,DEPT PHYSIOL,SHINJUKU KU,TOKYO 160,JAPAN
关键词
RECOVERIN; RECOVERIN-LIKE IMMUNOREACTIVITY; CALCIUM-BINDING PROTEIN; IMMUNOBLOT; CA-45; BLOT; IMMUNOHISTOCHEMISTRY;
D O I
10.1016/0006-8993(92)90676-Z
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Mouse brain was found by immunoblot analysis to have a protein of M(r) 23,000 (P23k) that was clearly different from recoverin and was labeled with an antiserum rasied against the NH2-terminus of recoverin. P23k could not be detected by an antiserum raised against the COOH-terminus of recoverin. Blots with Ca-45 demonstrated that P23k bound Ca2+ and had a Ca2+-dependent membrane-binding property Immunohistochemical studies provided the specific distribution of P23K in cerebral cortex and cerebellum.
引用
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页码:350 / 353
页数:4
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