SIMULTANEOUS IMMUNOSTAINING METHOD FOR LOCALIZATION OF BROMODEOXYURIDINE AND CALCITONIN GENE-RELATED PEPTIDE

被引：17

作者：

MONTUENGA, LM

SPRINGALL, DR

GAER, J

MCBRIDE, JT

POLAK, JM

机构：

[1] ROYAL POSTGRAD MED SCH,DEPT HISTOCHEM,DU CANE RD,LONDON W12 0NN,ENGLAND

[2] ROYAL POSTGRAD MED SCH,DEPT SURG,LONDON W12 0NN,ENGLAND

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1992年 / 40卷 / 08期

关键词：

5'-BROMODEOXYURIDINE; CALCITONIN GENE-RELATED PEPTIDE; LUNG; ENDOCRINE CELLS; IMMUNOHISTOCHEMISTRY; DOUBLE IMMUNOSTAINING METHOD;

D O I：

10.1177/40.8.1377732

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A new simultaneous double immunostaining method has been optimized to localize the DNA synthesis marker bromodeoxyuridine (BrdU) and calcitonin gene-related peptide (CGRP) in endocrine cells of Bouin's-fixed, paraffin-embedded rat lung. Nuclease pre-treatment before immunostaining is compatible with optimal tissue morphology and CGRP antigenicity preservation. Nickel-enhanced development of avidin-biotin-peroxidase staining is used to show CGRP immunoreactivity in black and alkaline phosphatase-anti-alkaline phosphatase is applied to demonstrate incorporated BrdU in red. The present methodology could be useful for studies requiring detection of incorporated BrdU in cells producing regulatory peptides or other labile antigens.

引用

页码：1121 / 1128

页数：8

共 40 条

[1] OCCURRENCE, DISTRIBUTION AND ONTOGENY OF CGRP IMMUNOREACTIVITY IN THE RAT LOWER RESPIRATORY-TRACT - EFFECT OF CAPSAICIN TREATMENT AND SURGICAL DENERVATIONS [J].