A NOVEL LIPOSOME-BASED ELECTROCHEMICAL BIOSENSOR FOR THE DETECTION OF HEMOLYTIC MICROORGANISMS

被引：19

作者：

KIM, HJ ^{[1
]}

BENNETTO, HP ^{[1
]}

HALABLAB, MA ^{[1
]}

机构：

[1] UNIV LONDON KINGS COLL,DIV LIFE SCI,MICROBIOL GRP,LONDON W8 7AH,ENGLAND

来源：

BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 06期

关键词：

D O I：

10.1007/BF00160823

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A biosensor strategy for rapid amperometric detection of organisms was investigated in which a redox mediator was entrapped within liposomes. The selective release of mediator by haemolytic bacteria, followed by signal generation, confers differentiation between haemolytic and non-haemolytic species. The potential of this approach is illustrated by results for various strains of Listeria monocytogenes, Listeria welshimeri and Escherichia coli.

引用

页码：389 / 394

页数：6

共 12 条

[1]

BENNETTO HP, 1987, BIOSENSORS FUNDAMENT, P291

[2]

BROCK TD, 1988, BIOL MICROORGANISMS, P315

[3] LISTERIA-MONOCYTOGENES MUTANTS LACKING PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C ARE AVIRULENT [J].

CAMILLI, A ;

GOLDFINE, H ;

PORTNOY, DA .

JOURNAL OF EXPERIMENTAL MEDICINE, 1991, 173 (03) :751-754

[4]

COSSART P, 1989, MOL BIOL MED, V6, P463

[5] LISTERIA-MONOCYTOGENES, A FOOD-BORNE PATHOGEN [J].

FARBER, JM ;

PETERKIN, PI .

MICROBIOLOGICAL REVIEWS, 1991, 55 (03) :476-511

[6] HEMOLYSIN FROM LISTERIA - BIOCHEMISTRY, GENETICS AND FUNCTION IN PATHOGENESIS [J].

GOEBEL, W ;

KATHARIOU, S ;

KUHN, M ;

SOKOLOVIC, Z ;

KREFT, J ;

KOHLER, S ;

FUNKE, D ;

CHAKRABORTY, T ;

LEIMEISTERWACHTER, M .

INFECTION, 1988, 16 :S149-S156

[7] LEVELS OF LISTERIA-MONOCYTOGENES HEMOLYSIN ARE NOT DIRECTLY PROPORTIONAL TO VIRULENCE IN EXPERIMENTAL INFECTIONS OF MICE [J].

KATHARIOU, S ;

ROCOURT, J ;

HOF, H ;

GOEBEL, W .

INFECTION AND IMMUNITY, 1988, 56 (02) :534-536

[8]

LASIC DD, 1993, LIPOSOMES PHYSICS AP

[9] FUNCTIONAL-CHARACTERIZATION OF A CLONED HEMOLYSIN DETERMINANT FROM ESCHERICHIA-COLI OF HUMAN-ORIGIN, ENCODING INFORMATION FOR THE SECRETION OF A 107K POLYPEPTIDE [J].

MACKMAN, N ;

HOLLAND, IB .

MOLECULAR & GENERAL GENETICS, 1984, 196 (01) :129-134

[10] ESCHERICHIA-COLI HEMOLYSIN PERMEABILIZES SMALL UNILAMELLAR VESICLES LOADED WITH CALCEIN BY A SINGLE-HIT MECHANISM [J].

MENESTRINA, G .

FEBS LETTERS, 1988, 232 (01) :217-220

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