GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC AND TANDEM MASS-SPECTROMETRIC INVESTIGATIONS OF SYNTHETIC AMINO-ACID CONJUGATES OF JASMONIC ACID AND ENDOGENOUSLY OCCURRING RELATED-COMPOUNDS FROM VICIA-FABA L

Some partial synthetic amino acid ((S)‐alanine, (S)‐leucine, (S)‐isoleucine, (S)‐aspartic acid, (S)‐glutamic acid, (S)‐phenylalanine, (S)‐ and (R)‐valine) conjugates of the plant growth regulator (‐)‐jasmonic acid (JA) were investigated as methyl esters by capillary gas chromatography/mass spectrometry. By gas chromatographic data (retention indices) as well as by mass spectral behaviour all these substances can be differentiated unequivocally. In addition, the distinction between the leucine and isoleucine JA conjugates was carried out by mass‐analysed ion kinetic energy spectroscopy (tandem mass spectrometry). Based on this some naturally occurring (S)‐isoleucine conjugates of (‐)‐JA, (+)‐7‐iso‐JA and related compounds as well as the (S)‐leucine and (S)‐phenylalanine conjugates of (‐)‐JA could be identified from extracts of the broad bean Vicia faba L. Copyright © 1990 John Wiley & Sons, Ltd.