SIMPLE METHOD FOR VISUALIZATION OF THE ISLETS IN FIXED BUT OTHERWISE INTACT PANCREAS

被引：13

作者：

KRAMLINGER, KG

MAYRAND, RR

LIFSON, N

机构：

[1] Department of Physiology, University of Minnesota Medical School, Minneapolis, MN

来源：

STAIN TECHNOLOGY | 1979年 / 54卷 / 03期

关键词：

D O I：

10.3109/10520297909112651

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A simple, inexpensive, reproducible method is described for prominently displaying the islets of Langerhans. The method consists of sequential arterial perfusion of the organ of the sacrificed animal with saline, formalin, hematoxylin, and water, followed by clearing in methyl salicylate. The procedure should be useful whenever islet tissue needs to be quantitatively distinguished from nonislet tissue and fixation is allowable. © 1979 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：159 / 162

页数：4

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